ISHS Acta Horticulturae 425: II International Pineapple Symposium
EFFECTS OF BENZYLAMINOPURINE (BAP) ON IN VITRO PROLIFERATION OF PINEAPPLE (ANANAS COMOSUS (L.) MERR.) |
| Authors: | W.A.B. de Almeida, A.P. de Matos, A. da S. Souza |
| DOI: | 10.17660/ActaHortic.1997.425.25 |
| Abstract:
This study was carried out in the Biotechnology Laboratory of the National Research Center for Cassava and Tropical Fruits (CNPMF) / Brazilian Organization for Agricultural Research (EMBRAPA), aiming to adjust a tissue culture technique for pineapple lateral buds, by determining the most adequate BAP concentration for maximum micropropagation rate, and by enabling the production of healthy pineapple plantlets, assuring genetic fidelity of the varieties ‘Primavera’ and ‘Pérola’. Explants were transferred to MS medium supplemented with AIA (2.0 mg l-1), sucrose (40.0 g l-1) and BAP (0.0, 1.0, 2.0, 3.0, 4.0, and 5.0 mg l-1) and kept for 3 months under 16 h photoperiod (1.500 lux) and 28±2°C. Plantlets were then transferred to MS medium supplemented with sucrose (40.0 g l-1), agar (7.0 g l-1) and BAP (0.0, 1.0 and 2.0 mg l-1) in order to induce proliferation, and thereafter to rooting medium with no growth regulator. Results showed that higher multiplication rates for ‘Pérola’ were obtained with BAP concentrations of 2.0 and 1.0 mg l-1 for the establishment and proliferation stages respectively, while for ‘Primavera’ the best results were obtained with BAP concentrations of 3.0 and 2.0 mg l-1 respectively. | |
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