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ISHS Acta Horticulturae 424: II International Rose Symposium

USE OF FLOW CYTOMETRY FOR THE RAPID DETERMINATION OF PLOIDY LEVEL IN THE GENUS ROSA

Authors:   Y. Jacob, C. Teyssier, S. Reynders-Aloisi, S.C. Brown
Abstract:
Ploidy levels of more than 150 rose genotypes were assessed by flow cytometry. The studied genotypes are representative of genus Rosa and were maintained in a collection at Fréjus. An approach by flow cytometry enabled rapid characterisation of somatic material in terms of ploidy level. It was possible to confirm or to infirm bibliographical data and even to identify mistakes in terms of name or label. The characterisation of ploidy level is interesting for the study of a complex and vast genus such as Rosa for which no exhaustive identification key exists. Due to the variability between studied genotypes, flow cytometry has not always permitted to determine precisely the ploidy level of some genotypes among botanical species.

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