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ISHS Acta Horticulturae 424: II International Rose Symposium

DNA MARKERS IN ROSE AND THEIR USE FOR CULTIVAR IDENTIFICATION AND GENOME MAPPING

Authors:   R. Ballard, S. Rajapakse, A. Abbott, D.H. Byrne
Abstract:
We have identified DNA markers that can be used to identify rose cultivars. By comparing RFLP and RAPD patterns, we have been able to characterize 20 of the 22 cultivars examined to date. Our results suggest that both RFLP and RAPD markers are useful for rose cultivar identification and may provide means for protecting patent rights to new cultivars. Additionally, we have initiated a genetic mapping program to identify molecular markers associated with blackspot resistance in roses. Preliminary RAPD analysis of the parental rose plants of the crosses established to study blackspot resistance in roses shows that a high degree of genetic polymorphism exists between all the resistant amphidiploids and susceptible tetraploid parent rose cultivars. This demonstrates that progeny produced between any two resistant and susceptible parents would be suitable for genetic map construction.

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