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ISHS Acta Horticulturae 424: II International Rose Symposium

CHARACTERISATION OF GENETIC DIVERSITY IN GENUS ROSA BY RANDOMLY AMPLIFIED POLYMORPHIC DNA

Authors:   S. Reynders-Aloisi, P. Bollereau
Abstract:
Molecular polymorphism revealed by RAPD (Randomly Amplified Polymorphic DNA) was studied on a set of botanical rose trees with 10 mer Operon primers. A very large variability is shown. The RAPD profiles were analysed in terms of presence/absence of the various fragments The individual/fragment table is then studied by canonical discriminant analysis and cluster analysis. The relative position of individuals on the first canonical axes and the groups observed in cluster analysis are interpreted in comparison with membership of botanical sections. The case of some individuals whose denomination and/or classification is doubtful is compared with results from the morphological approach and from the ploïdy assessment by flow cytometry.

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