ISHS Acta Horticulturae 424: II International Rose Symposium
DETECTION AND DETERMINATION OF PATHOGENIC AGROBACTERIUM OF ROSES WITH PCR |
| Authors: | S. Pionnat, X. Nesme, Y. Dessaux, C. Poncet |
| Abstract:
Crown-gall, caused by pathogenic Agrobacterium, is one of the most worrying rose diseases. The real cause of pathogenicity is the Ti plasmid (Tumor Inducing), located in its cytoplasm. During infection, part of this plasmid, the T-DNA, is transferred to the genome of a plant cell and causes the proliferation of the host cells and the formation of galls. The prerequisite for the control of crown gall is to have a reliable detection method of pathogenic Agrobacterium in order to select healthy plants. Therefore, we have established a method for the analysis of bacterial strains isolated from galls of roses of various origins by Polymerase Chain Reaction. Amplification was performed on three plasmidic sequences, located in the conserved virulence genes, and in the T-DNA (on tmr and tms genes). After analysis, the banding patterns of different strains were found to correspond to the biological tests for the determination of pathogenicity and the biochemical tests for the determination of the genus. Therefore, PCR was integrated as a determination tool in isolation methodology of pathogenic Agrobacterium from galls, and is now currently used in the laboratory. | |
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