ISHS Acta Horticulturae 423: II Workshop on Pollination
THE SELF-INCOMPATIBILITY GENE IN APPLE AND DETERMINATION OF THE S-GENOTYPE OF APPLE CULTIVARS BY PCR |
| Authors: | W. Broothaerts, L. Verdoodt, J. Keulemans, G.A. Janssens, W.F. Broekaert |
| Keywords: | Allele-specific PCR, Malus X domestica, pistil, pollination, ribonuclease, S-allele, S-gene |
| Abstract:
Pollen-pistil interactions in apple (Malus X domestica Borkh.) are controlled by a gametophytic self-incompatibility (SI) system that prevents self-pollen from reaching the ovary for fertilization. Several alleles of the highly polymorphic SI gene (S-gene) in apple have been cloned and characterized. Comparison of the derived amino acid sequences of S-alleles in apple reveals that the protein structure is composed of alternating conserved and variable sequence regions. Allele-specific PCR primers, designed to anneal within the variable sequence domains, were used to analyse the presence of the corresponding S-alleles in genomic DNA from various cultivars. Here we report on recent data on the S-genotypes of old and current apple cultivars, determined by PCR typing of the S-alleles. | |
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