PRODUCTION OF TRANSGENIC PLANTS OF CHRYSANTHEMUM MORIFOLIUM RAMAT WITH THE GENE OF BAC. THURINGIENSIS δ-ENDOTOXIN

Authors:	S.V. Dolgov, T.U. Mityshkina, E.B. Rukavtsova, Y.I. Buryanov
Abstract: Based on the developed regeneration methods, a genetic transformation of Chrysanthemum varieties by vector constructions with the gene of Bac. thuringiensis -endotoxin was performed. Transformation of Bornholm by cointegrative vector pGV3850 with the -endotoxin gene under TR-1' promoter did not result in production of plants stably growing on selective media with the high content of antibiotics. Transformation by a binar vector system based on the armed strain A281 and plasmid pBTP41 with the -endotoxin gene under promoter 35S allowed production of 2 lines of White Harricone plants with incorporation of the -endotoxin gene into the genome supported by blot hybridization according to Sauthern. During the active growth in a greenhouse, the transgenic plants were not visibly injured by web tick in the absence of chemical treatment.
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