ISHS Acta Horticulturae 419: I International Symposium on Pistachio
ISOENZYMIC IDENTIFICATION OF PISTACIA SPECIES |
| Authors: | M. Rovira, I. Batlle, M. Romero, F.J. Vargas |
| Keywords: | Pistacia species, isoenzyme, gel electrophoresis, identification |
| DOI: | 10.17660/ActaHortic.1995.419.43 |
| Abstract:
Six Pistacia species (P. atlantica, P. integerrima, P. khinjuk, P. palaestina, P. terebinthus and P. vera), were studied for ten isoenzyme systems. Starch gel electrophoresis of leaf extracts was used to analyse the variability of aconitase (ACO), esterase (EST), glutamate oxalacetate transaminase (GOT), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6PGD), glucose-6-phosphate isomerase (GPI), phosphoglucomutase (PGM) and shikimate dehydrogenase (SKD). Zymograms of four systems (GOT, MDH, GPI and PGM) showed distinct banding patterns for different species which was useful for their characterization, GPI being the most suitable. SKD was variable for all the species, but useless for their identification. The most polymorphic species was P. atlantica for six isoenzymes (EST, GOT, GPI, MDH, PGM and SKD), followed by P. vera for five (ACO, EST, GPI, PGM and SKD); P. palaestina for four (GOT, GPI, PGM and SKD); P. integerrima for three (ACO, PGM and SKD); and P. khinjuk and P. terebinthus for two isoenzymes (GOT and SKD). | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
