ISHS Acta Horticulturae 419: I International Symposium on Pistachio
ASPECTS OF IN VITRO CULTURE OF PISTACIA TEREBINTHUS AND PISTACIA VERA |
| Authors: | S. Gannoun, S.M. Lionakis, D. Gerasopoulos |
| Keywords: | P. terebinthus, P. vera, micropropagation, in vitro culture, browning, etiolation, rejuvenation |
| Abstract:
Browning of Pistacia terebinthus explants in vitro - taken from 2, 4 and 29-year-old plants was successfully controlled by adding in the medium 100 mg/l ascorbic acid and by immersing the explants prior to culture for 30 minutes in a aqueous solution containing 10g/l polyvinylpyrrolidone (PVP). However, the browning was not controlled when explants were taken from 8 and 26 years old Pistacia vera plants. Explants from 2 and 4-year-old P. terebinthus plants showed significantly higher proliferation rate in vitro than explants from 1 year old plants or 29-year-old rejuvenated plants. Single node shoot segment explants from 29 years old rejuvenated P. terevinthus plants produced in vitro significantly more shoots than shoot tip explants from 1-year-old plants. Etiolation pretreatement of plant material taken from 1, 2 and 4-year-old P. terebinthus plants did not affect significantly shoot proliferation in vitro. High shoot proliferation was achieved from explants taken from 1-month-old P. vera seedlings, while internal contamination was a serious problem for the establishment of explants particularly for those taken from adult, 8 and 26-year-old, P. vera plants. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
