ISHS Acta Horticulturae 418: III International Symposium on Artificial Lighting in Horticulture
EFFECTS OF RED LIGHT ON THE GROWTH AND MORPHOLOGY OF POTATO PLANTLETS IN VITRO: USING LIGHT EMITTING DIODES (LEDS) AS A LIGHT SOURCE FOR MICROPROPAGATION |
| Authors: | Y. Miyashita, T. Kimura, Y. Kitaya, C. Kubota, T. Kozai |
| Abstract:
Red light emitting diodes (LEDs, peak wavelength: 660 nm) and white fluorescent lamps were used as light sources for growth of potato plantlets in vitro. Red (630–690 nm) photon flux (R-PF) on the empty culture shelf were adjusted at 11, 15, 28, 47 or 64 μmol m-2s-1. Photosynthetic photon flux (PPF) on the empty culture shelf was adjusted at 100 μmol m-2s-1 in all the treatments. Potato plantlets were cultured on Murashige & Skoog (1962) medium with or without sugar at an air temperature of 25°C and a photoperiod of 16 h. Shoot length and chlorophyll concentration of the plantlets increased with increasing R-PF, while there were no significant differences in dry weight and leaf area of the plantlets regardless of R-PF levels. Red light affected the morphology rather than the growth rate of potato plantlets in vitro. Red LEDs can be used for controlling plantlet morphology in micropropagation. | |
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