ISHS Acta Horticulturae 415: VIII International Asparagus Symposium
PHLOROGLUCINOL AND TRYPTONE ENHANCE IN VITRO ROOTING AND SURVIVAL RATE OF ASPARAGUS NODAL SECTIONS |
| Authors: | D. C.N. Chang, K.H. Peng |
| Abstract:
Rooting percentage of Tainan Selection #3 (TS #3) asparagus nodal sections in MS medium with NAA, kinetin and 3% sucrose was only 1.7%. Tryptone (250 mg/l), phloroglucinol (PG; 162 mg/l) and ancymidol (1.28 mg/l) were added to a modified MS medium containing NAA (0.15 mg/l), kinetin (0.15 mg/l) and 6% sucrose to investigate their potential for increased rooting of in vitro asparagus cultures. The asparagus rooting percentage in control medium was 18%, while in medium with tryptone, PG or ancymidol, the rooting percentages increased to 41%, 59% and 63% respectively. In a combination of tryptone or PG with ancymidol, the in vitro rooting of asparagus reached 73–78%. In addition to increasing the frequency of rooting, the presence of PG or tryptone significantly increased shoot number and root length. In contrast, ancymidol reduced shoot length, but significantly increased the diameter of shoots and roots, and number of shoots per plantlet. Transplanting survival rate of micropropagated asparagus plantlets was influenced by temperature, plastic bag covering and inoculation with Glomus spp. which induce vescicular-arbuscular mycorrhizal associations. | |
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