ISHS Acta Horticulturae 415: VIII International Asparagus Symposium
AERIAL CROWN-LIKE BODY FORMATION THROUGH NODAL SEGMENT CULTURE IN ASPARAGUS. |
| Authors: | H. Araki, S. Watanabe, T. Harada, T. Yakuwa |
| Abstract:
An aerial crown-like body was often formed at the node in asparagus. It was similar to the underground crown forming at the basal portion of the seedling in histological observation, because of the numerous crown buds and vascular bundles with no fixed orientation. Aerial crown-like body was accelerated by culturing the nodal shoot segments of young asparagus seedlings in modified MS medium supplemented with 0.5–2 mg·liter-1 ancymidol and 0.1–0.2 M sucrose. In the medium supplemented with 2 mg·liter-1 ancymidol and 0.1 M sucrose, aerial crown-like body formation and crown bud induction were very frequent. Aerial crown-like body formation was completely suppressed in the presence of 1–10 mg·liter-1 gibberellin (GA3), and GA3 made the lateral buds develop lateral shoots. Vigorous rooting was accelerated by transferring the nodal segments with aerial crown-like body to the basal medium. The plantlets obtained were successfully transplanted to soil. The resulting aerial crown-like body was induced by inhibiting GA activity, and its formation was effective for propagation through tissue culture. | |
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