ISHS Acta Horticulturae 412: I International Symposium on Solanacea for Fresh Market
ARTIFICIAL SEEDS OF PEPPER SOMATIC EMBRYOS |
| Authors: | S. Büyükalaca, F. Mavituna |
| Keywords: | Capsicum annuum, encapsulation, somatic embryogenesis |
| Abstract:
Artificial seeds are a potential method for delivering somatic embryos to the field. Mature somatic embryos were encapsulated in calcium alginate gel using different concentrations of sodium alginate (2–6%) and calcium chloride (25–100 mM) in order to determine the effect of rigidity of bead on the conversion frequency of somatic embryos. MS medium supplemented with 3% sucrose was used for germination. Variability was obtained between sodium alginate and calcium chloride concentration with respect to % germination. Plant regeneration frequency decreased with an increase in sodium alginate concentration above 3%. 3% sodium alginate and 75 mM calcium chloride gave the best conversion and optimum bead rigidity and shape. In vitro and in vivo conversion of naked and encapsulated embryos were compared. | |
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