ISHS Acta Horticulturae 410: II International Cherry Symposium
IN VITRO MASS PROPAGATION OF ELEVEN PRUNUS MAHALEB ECOTYPES |
| Authors: | G. Dradi, G. Vito, A. Standardi |
| Abstract:
Eleven ecotypes of Prunus mahaleb, promising as rootstocks for two cultivars of sweet cherry, were submitted to in vitro culture to test if micropropagation could be used in commercial laboratories for their rapid production. Satisfactory stabilization of aseptic culture was achieved in 30–50 days (according to ecotype), using 2 mm long bud tips as initial explants, cultured on agarized medium containing SH macronutrients, MS micronutrients and vitamins, 0.5 mg/l BAP, 0.5 mg/l GA3, 0.01 mg NAA, 10 mg/l citric acid, 2 mg/l glycine, 30 g/l sucrose and 7 g/l purified agar. After three subcultures of 21 days each, on four different proliferating media, the average multiplication rate was 3.4 on medium containing SH macronutrients, MS micronutrients, and CH vitamins, 0.8–3.0 mg/l BAP and 0.01 mg/l NAA. Nevertheless, proliferation was greatly influenced by ecotype. In fact, among the 11 promising rootstocks of Prunus mahaleb, multiplication rates on the same medium ranged from 1.5 to 4.5. The rooting percentages of eight ecotypes ranged from 0 to 88%, depending on ecotype and IBA concentration in the rooting media. Usually, satisfactory results were obtained with media containing 50 mg/l activated charcoal and 0.8–3.0 mg/l IBA. | |
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