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ISHS Acta Horticulturae 407: ISHS Brassica Symposium - IX Crucifer Genetics Workshop

CHIMERA SYNTHESIS AND PROPAGATION SYSTEMS BY IN VIVO AND IN VITRO GRAFT METHODS IN BRASSICA

Authors:   S. Oguni, Y. Hirata, T. Kan, M. Kita, H. Horiuchi, T. Noguchi
Keywords:   Grafting, Tissue culture, Red cabbage, Komatsuna, Rape
DOI:   10.17660/ActaHortic.1996.407.27
Abstract:
In vivo and in vitro chimera synthesis methods were established for Brassica crops. As in vivo grafting method was not efficient to obtain sufficient number of chimeras, approach-grafted seedling culture (AGSC) method was finally adopted in synthesizing interspecific Brassica chimeras. Vegetative progenies are stably obtained by the method. Interspecific chimeras between B. campestris-B. oleracea produced mericlinal types, while, interspecific chimeras between B. napus-B. oleracea produced periclinal types. The mixed type of chimera is best for maintaining the various types of chimeras in vegetative propagation systems from chimeral plants.

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