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ISHS Acta Horticulturae 405: VI International Symposium on Postharvest Physiology of Ornamental Plants

EXPORT OF 14C-SUCROSE, 3H-WATER, AND FLUORESCENT TRACERS FROM GLADIOLUS FLORETS TO OTHER PLANT PARTS ASSOCIATED WITH SENESCENCE

Authors:   K. Yamane, Y. Kotake, T. Okada, R. Ogata
Keywords:   cut flower, postharvest physiology, wilting
Abstract:
Solutions of 14C-sucrose and 3H-water were fed to full unfolded perianths of lower florets on cut gladiolus spikes. When the fed perianths completely wilted, substantial quantities of 14C and 3H were recovered from the other perianths, ovaries, leaves and stalks of the spikes.

After feeding solutions of fluorescein (FL) and 6(5) carboxyfluorescein [6(5)CF] to unfolded perianths of the first florets, transverse sections were prepared and observed with a fluorescence microscope. When the fed perianths began to wilt, FL appeared in phloem region of the ovary of the first and fifth floret. 6(5)CF appeared in the perianth margin of the fifth floret. It appeared in xylem region and tissues out of vascular bundles in the ovary of the first floret.

The results indicate that export of sucrose, water and other substances from perianths to other plant parts occurred in association with wilting of the perianths. These substances could be translocated through not only phloem region but also xylem region and tissues out of vascular bundles.

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