ISHS Acta Horticulturae 405: VI International Symposium on Postharvest Physiology of Ornamental Plants
PROLONGATION OF VASE LIFE OF CUT ROSES VIA INTRODUCTION OF GENES CODING FOR ANTIBACTERIAL ACTIVITY. SOMATIC EMBRYOGENESIS AND AGROBACTERIUM-MEDIATED TRANSFORMATION. |
| Authors: | F.H.M. Derks, A.J. van Dijk, C.H. Hänisch ten Cate, D.E.A. Florack, L.A.M. Dubois, D.P. de Vries |
| Abstract:
A reproducible regeneration method via somatic embryogenesis was developed for various rose cultivars. In vitro induced roots were cultured on medium with high concentrations of auxins followed by culture on hormone-less medium. Embryogenic callus with somatic embryos was obtained, which resulted into normal plants. Transformation of embryogenic callus of 3 cultivars was performed with Agrobacterium tumefaciens, harbouring a binary vector containing a kanamycin resistance gene, a | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
-glucuronidase (GUS) gene with an intron and a gene coding for the antibacterial cecropin B. Transgenic callus was obtained for all binary vectors used.
Transgenic somatic embryos were induced on transgenic callus of cultivar ‘Sonia’.
