ISHS Acta Horticulturae 403: International Symposium on Cultivar Improvement of Horticultural Crops. Part 2: Fruit Crops
EXPERIMENT ON THE CRYOPRESERVATION OF APPLE SHOOTS IN VITRO |
| Authors: | M. Wenhuang, T. Sanada |
| Keywords: | apple, hardening at low temperature, pretreatment, preliminary freezing, cryopreservation, survival rate |
| Abstract:
The results of experiment on cryopreservation of shoots of apple varieties Fuji, etc. showed that the survival rates of plantlets hardening at 5°C for 15, 35, and 60 days before cryopreservation were 0oC, 88.8%, and 27.0, respectively. Survival rates of shoots cultured on the medium containing 1.5%, 3%, and 6% sucrose were 80.0%, 85.7%, and 46.2%, respectively. Survival rates of shoots cultured on the medium containing 1 x 10-7, 5 x 10-7, 1 x 10-6, BA were 77.8%, and 70.0%, respectively. When the shoots were transferred to MS medium containing 2.5% DMSO for 4 days after hardening at low temperature, survival rate was 90.0%. When the shoots were cultured for 8 days or transferred to MS medium containing 5% DMSO for 8 and 4 days, survival rates were 45.5%–80.0%. Survival rates treated at 5oC in pretreatment solution containing 5% DMSO+14% sucrose for 3.18 48 h were 35.7%, 85.7% and 33.3%, respectively. Survival rates of shoots treated in pretreatment solution without DMSO or with added 5% DMSO were over 80% and survival rates of shoots treated in pretreatment solution with added 10% DMSO were below 52.0%. The highest survival rate was 90.6% and the highest regenerating rate was 54.6% for Fuji apple. The highest survival rates were 83.0% and 100% and the highest regenerating rates were 16.7% and 23.1% for Gala and MM106. Survival rate and regenerating rate for triploid apple variety Mutsu were relatively low. | |
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