ISHS Acta Horticulturae 403: International Symposium on Cultivar Improvement of Horticultural Crops. Part 2: Fruit Crops
CITRUS BIOTECHNOLOGY RESEARCH IN CHINA |
| Authors: | X. Deng, Z. Deng, X. Ye, W. Zhand |
| Keywords: | citrus, Biotechnology, Breeding, Protoplast culture, somatic hybrid |
| Abstract:
Citrus biotechnology research has been conducted since early of 1970's in China. This paper reviews its development, and the progress achieved since then. Plant regeneration systems for anther culture, endosperm culture, and young embryo culture were established prior to 1980. Protoplast derived plants of 8 cultivars (species) including orange, tangerine and kumquat were obtained in 1988. This system enhanced the selection of a salt-tolerant line of sweet orange, and the regeneration of intergeneric and interspecific somatic hybrid plants. 2 of the 4 somatic hybrid combinations obtained in the past 2 years have been topworked for the evaluation of their agronomic performance. These studies were aimed at overcoming the barriers encountered in the conventional breeding, such as polyembryony, heterozygosity, and sterility. The utilization of shoot tip grafting technique facilitate the virus-free program of citrus in China. The result of a chromosome variation study of nonembryogenic calli of citrus provides a new channel for obtaining auto-polyploid, chromosome counts during the regeneration of fused protoplasts revealed that some of the combinations which were very difficult to grow out plantlets exhibited chromosome elimination phenomena. Genetic transformation and RFLP analyses programs have been initiated recently, and some progress has been achieved. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
