ISHS Acta Horticulturae 402: International Symposium on Cultivar Improvement of Horticultural Crops. Part 1: Vegetable Crops
EFFECTS OF WHOLE RI T-DNA AND AUXIN GENES ALONE ON ROOT INDUCTION AND PLANT PHENOTYPE OF CHINESE CABBAGE |
| Authors: | H. Yuke, W. Jingyou, W. Zhiiming, X. Zhihong, G. Zhenhui |
| Keywords: | Agrobacterium, Auxin genes, Chinese cabbage, Root induction |
| Abstract:
Agrobacterium strain LBA9402Bin19 embraces pRi1855 and binary vector pBin19 and the strain LBA4404auxB harbours pTi4404 and binary vector pBin19 containing a set of auxin genes consttructed in our laboratory. We used these two strains for genetic transformation of Chinese cabbage (Brassica pekinensis). The cut or pierced cotyledons were inoculated with suspension of these two strains, giving rise to transformed roots and kanamycin-resistant callus. The transformants were identified and cut into 1 cm long segments or small callus clusters and cultured on MS solid medium containing 3 mg/l kinetin. Plantlets were differentiated from them after 40 days and so on. Whole Ri T-DNA induced hairy roots and abnormal plants since plants derived from LBA9402Bin19-transformed roots hand shrunk leaves and partly-plagiotropical roots. Auxin genes alone procuced normal phenotype in that mostt plant derived from LBA4404auxB-incited callus showes the phenotype dentical to normal plants. Rherefore, auxin genes were more desirable for appearance of transsgenic plants with higher auxinconentration and normal phenotype, compared with whole T-DNA. When the leaves were cut from transgenic plants of two sourace of the leaf explants. In the absence of hormone, the leaves of some transgenic plants tened to be upward and innerward slighlty. | |
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