ISHS Acta Horticulturae 402: International Symposium on Cultivar Improvement of Horticultural Crops. Part 1: Vegetable Crops
IDENTIFICATION OF HOMOZYGOTES DERIVED FROM ANTHERS OF ASPARAGUS (ASPARAGUS OFFICINALIS L.) WITH ISOZYME MARKERS |
| Authors: | Q. Deyou, Z. Weiyan |
| Keywords: | Asparagus officinalis L., anther culture, isozyme gene markers, homozygote, all-male lines |
| Abstract:
Eleven enzymes of Asparagus (Asparagus officinalis L.) were examined on polyacrylamide gels to identify polymorphic Loci useful as genetic markers. Two polymorphic Loci were discovered, one a root esterase (EST-1) and the other a shikmic acid dehydrogenase (SKD-1). Segregation of these two polymorphic Loci was examined by the test cross and was found to be consistent with a simple Mendelian ingeritance. Both these two genetic markers were stable allelic Loci and useful tools to idnetify plants derived from microspores, electrophoretic and cytological analysis showed that haploids, doubled haploids and homozygous tetraploids were 5%, 8.33%, 6.67% respectively among anther-derived plants, all these hozygotes are useful for breeding all-male lines of Asparagus. | |
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