ISHS Acta Horticulturae 402: International Symposium on Cultivar Improvement of Horticultural Crops. Part 1: Vegetable Crops
ANTHER CULTURE OF SEVERAL SWEET AND HOT PEPPER GENOTYPES |
| Authors: | X. Qin, G.L. Rotino |
| Abstract:
Four F1 hybrids and 13 cultivars or breeding lines of sweet and hot pepper were grown in the greenhouse and used as anther donor genotypes. Anther culture was performed following the method described by Dumas de Vaulx et al. (1981) with modifications regarding the growth regulators (0.5 mg/l 2,4D and 0.5mg/l KIN or 0.5 mg/l 2.4D and 0.5 mg/l 6-BAP). Results showed that in some case embryos were obtained only in medium containing 6-BAP. A total of 268 embryos were derived from 2937 in vitro cultured anthers of 15 genotypes and 181 mature plants were obtained from 12 genotypes. Among the regenerated androgenetic plants about and equal number of haploid and diploid plants were found. | |
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