ISHS Acta Horticulturae 402: International Symposium on Cultivar Improvement of Horticultural Crops. Part 1: Vegetable Crops
IN VITRO PROPAGATION OF CHINESE CABBAGE FOR SEED PRODUCTION |
| Authors: | L. Xiaoou, S. Rifei, W. Feiyan, S. Jiagang, N. Xinke, L. Chunling, J. Zhongren, G. Yangdong, Y. Baojun |
| Keywords: | Brassica pekinensis, In Vitro Propagation, Self-incompatible line, Male sterile line, Hybrid seed production |
| Abstract:
Chinese cabbage F1 hybrids have been produced by using self-incompatible lines or genetic male sterile lines. However, it is difficult to produce SI lines and to hand-rogue of half male-fertile plants from male sterile parents. In order to overcome the problems, an in vitro propagation technique for Chinese cabbage F1 seed production has been developed. In this paper, the main technique and the practical examples of the in vitro propagation of Chinese cabbage for seed production have been elaborated. Among 9 experimental media, 88–13 was the best. The results of experiments with various combinations of NAA, IAA, 2,4-D, 6BA and KT showed that 6BA 1.0–5.0 mg/L and NAA 0.1–1.0 mg/L were the optimum components and concentrations. The best culture conditions were with the temperature of 18–24°C/day, 10–15 °C/night and 1 000–2 000 Lux for 10 hours a day with cold white fluorescent light. Seedlings were transported in plastic bags. Seedlings preserved in plastic bags for 3 days showed high survival rate. The optimum medium for transplantation was the mixture of vermiculite and garden soil or the mixture-of sand and garden soil. After transplanted, the seedlings were maintained at 10–25 °C and moisture was the key of survival. From 1989 to 1991, 6 F1 hybrids have been produced by using the new technique. In three years, the total experiment area were 0.25 ha.. 218.75 Kg F1 hybrid seeds were produced. The average yields were 875 Kg per ha.. Purity tests showed that the F1 hybrids produced by the new technique were highly pure and uniform. And the economic characters were better than those of F1 hybrids produced by normal methods. | |
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