ISHS Acta Horticulturae 402: International Symposium on Cultivar Improvement of Horticultural Crops. Part 1: Vegetable Crops
IMPROVED RATE OF CALLUS AND PLANTLET FROM ANTHER CULTURE OF ASPARAGUS (ASPARAGUS OFFICINALIS L.) |
| Authors: | S. Shuxing, Z. Daoqian, Z. Chenghe, L. Shixing |
| Abstract:
Asparagus anthers containing microspore in early-uninucleate to early-binucleate stages were successfully cultured on the ½MS medium (Except FeNa-EDTA) supplemented with 1.0 mg/L 2.4-D, 0.5–2 mg/L NAA and 0.5–2 mg/L BA for callus formation. The highest frequency of callus induction was 78.05%. Callus formation ability differed among individual plants. The organogenesis were induced when calli were subcultured to MS medium with 0.05–0.2 mg/L BA and 0.05–0.3 mg/L NAA. The highest frequency of stem and pseudo-leaf induction (76.96%) was obtained on the MS medium with 0.1 mg/L NAA and 0.1 mg/L BA, a few callus developed shoots and roots as well. Clustered shoots were cultured on the half strength MS (Except FeNa2-EDTA) medium with 0.1 mg/L NAA or 2 mg/L IBA and 1–2 mg/L IAA or 2 mg/L IBA and 0.1 mg 1 NAA or 1.5 mg/L IBA and 0.1 mg/L KT, the roots were initiated. The highest rooting percentage can be raised to 62.37% on ½ MS (Except FeNa-EDTA) with 0.1 mg/L NAA, the roots were normal. Hardened the regenerative plants in the culture flask with cotton plug in greenhouse with strong light for 15–20 days. The plantlets had normal pseudo-leaves, strong roots and strong resistance. The transplanting survival rate can be raised to 84.55%. | |
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