ISHS Acta Horticulturae 402: International Symposium on Cultivar Improvement of Horticultural Crops. Part 1: Vegetable Crops
EXPRESSION OF CUCUMBER MOSAIC VIRUS COAT PROTEIN CONFERS CROSS-PROTECTION IN TRANSGENIC TOMATO PLANTS |
| Authors: | Y. Rongchuang, X. Helin, L. Mingsheng, Y. Wengui, L. Chungui, W. Guang, P. Naisui, C. Zkhangliang |
| Keywords: | Agrobacterium tumefaciens, Leaf-disk transformation, CMV-cp gene, Tomato |
| Abstract:
A chimeric gene containing a cloned cDNA of the coat protein(cp) of cucumber Mosaic Virus (CMV) was transferred into tomato cells, and 42 transgenic tomato plants have been produced by mean of a novel leaf disk transformation with the help of a modified Ti plasmid of agrobacterium tumefaciens. The results of DNA Southern blot analysis confirmed that CMV-cp gene was stably integrated into the genome of transgenic plants. CMV coat protein was expressed in the plant cells tested by Dot blot. It was shown that the transgenic plants of tomato results in protection of these plants from infection by CMV. The rate of systemic infection of the virus is reduced or delayed compared with the control plants. The segregation of the Rl progeny of CMV resistance is a 3:1 ratio indicating a single insert inherited in a Mendelian fashion. | |
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