ISHS Acta Horticulturae 402: International Symposium on Cultivar Improvement of Horticultural Crops. Part 1: Vegetable Crops
A STUDY OF THE GENETIC PARAMETERS IN A WILD BRASSICA OLERACEA L. POPULATION* |
| Authors: | X. Qi, D.F. Marshall, B.V. Ford-Lloyd |
| Keywords: | Brassica oleracea, Electrophoresis, Phosphoglucoisomerases, Phosphoglucomutase, Mating system, Population genetics |
| Abstract:
Two well resolved, easily interpretable isozymes: phosphogluco-isomerases (PGI) and phosphoglucomutase (PGM) were analyzed. 498 individuals from 50 families of a wild Brassica oleracea population which was collected from the limestone sea cliff in Winspit, Dorset, England were assayed and scored for pgi-2 and pgm-2 isozyme loci. Various genetic parameters(tm, ts, F & pj) were estimated by running the multilocus estimation program (MLT Program) which was developed by Kermit Ritland. Three alleles, one common allele with frequency pc=0.905(0.018) and two rare alleles pa=0.047(0.012) & pb=0.048(0.013), were found at pgi-2. One common allele pb=0.547(0.026) and two less common alleles pa=0.176(0.023) & pc=0.277 (0.024) of pgm-2 were detected. These isozyme marker gene frequencies data are useful to the germplasm conservation and the ecologic aspects associated with this population. The valid estimation of outcrossing rate, tm=0.819 (0.072), indicates that about 20% of progenies are derived from selfing, about 80% are from outcrossing. It suggests that high outcross rate may be caused by the existence of self-incompatibility genes in the population, and this is important to store genetic diversity. Certain degree of selfing in the self-incompatibility population also have the evolutionary advantage for the survival of the population if the selection pressure was high. | |
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