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| Authors: | T. Matsuo, N. Kamigama, M. Saitoh |
| Keywords: | Astringent component, (+)-catechin, de-proteineizing agent, flavonoid, immobilized tannins, persimmon fruit, removal of iron ions and proteins, tannin |
Abstract:
In Japan, astringent persimmon fruits have been used as an important source of condensed tannins, which have various uses; as a de-proteinizing agent and a coating material to fibers, paper, and wood.
We have devised a simple method to immobilize high molecular weight tannins on the surface of polystyrene resin.
Such tannins were easily adsorbed on the polystyrene surface by hydrophobic interaction and then cross-linked with an acidic aldehyde.
The immobilized tannins (about 603 mg per 10 ml resin) obtained thus did not lose adsorption abilities for heavy metals, proteins and alkaloids.
A column (10 ml gel wet volume) packed with the kaki-tannin-immobilized HP-20 resin removed 102 mg of BSA, 60 mg of lysozyme, 92 mg of bovine hemoglobin at a flow rate of 200 ml/hr (SV=20), and 12.5 mg of Fe ion (SV=4) from aqueous solution.
The column is expected to be useful for removing some proteins from sake, Japanese rice wine, which cause haze during transportation, storage and marketing.
It is also effective for removing a small concentrations of iron ions from water in the food industry.
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