ISHS Acta Horticulturae 397: New Ornamental Crops and the Market for Floricultural Products
IN VITRO PROPAGATION OF THIN-LEAF SUNFLOWER (HELIANTHUS DECAPETALUS L.) |
| Authors: | T. Hosoki, K. Ohta, K. Inaba, M. Harisaki |
| Keywords: | Micropropagation, Node-culture, Perennial-sunflower, BA (N6-benzyladenin), IBA (indole-3-butyric acid) |
| Abstract:
In vitro propagation of thin-leaf sunflower (Helianthus decapetalus L.) was achieved by subculturing shoot sections with nodes in Murashige-Skoog (MS) medium supplemented with 0.1 mg N6-benzyladenine (BA) · liter-1. A multiplication rate of 4.9 per 22-day culture period was achieved, resulting in theoretically yielding 110.4 billion shoots from a shoot tip in a year. Shoots elongated from these in vitro cultured nodes could be rooted in MS medium with 0.1 mg indole-3-butyric acid (IBA) · liter-1. Plants produced were readily acclimatized and flowered in a greenhouse. | |
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