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ISHS Acta Horticulturae 396: Hydroponics and Transplant Production

INJURY OF ROOT TISSUE CAUSED BY A CHLORINE COMPOUND IN NUTRIENT SOLUTION MADE OF TAP WATER

Authors:   S. Date, K. Naba, K. Kobayashi, T. Namiki
Keywords:   Lactuca sativa L., Combined Chlorine, Root Injury, Cortex, Central Cylinder, TTC Reducing Activity, Hydroponics
Abstract:
Lettuce was cultured in the half strength Enshi-shohou nutrient solution containing 0.5ppm of residual chlorine and the progress of the root injury was studied with the passage of time. One hour after chlorine treatment, we found some wilting, 50% decline of TTC reducing activity of the root tissue, no coloring by TTC reducing reaction in the cortex and slight coloring in the central cylinder. Three hours after chlorine treatment, we found almost all plants wilting, more decline of TTC reducing activity of the root tissue, break down of epidermal cells and no coloring by TTC reducing reaction in the central cylinder. The TTC reducing activity of the root tissue and the coloring status by TTC reducing reaction in the root tissue remained unchanged until 36 hours after chlorine treatment. Twentyfour hours after chlorine treatment, we found break down of the cortex and central cylinder cells. It is concluded that the root injury with the combined chlorine occurred through rapid decline of physiological activity of the root tissue and following break down of the root cells.

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