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ISHS Acta Horticulturae 396: Hydroponics and Transplant Production

INTEGRATED ANALYSIS OF NITROGEN STATUS IN CUCUMBER PLANTS GROWN IN SOLUTIONS OF DIFFERENT IONIC STRENGTH

Authors:   S.H. Ha, G.C. Chung
Keywords:   Cucumber (Cucumis sativus L.), Nitrate, Petiole sap, Nitrate reductase, Nitrogen concentration
Abstract:
In order to collect information on the nitrogen status in cucumber plant, nitrate concentration in petiole sap, in vivo nitrate reductase activity(NRA) and total nitrogen content in leaf blade were analyzed. Since cucumber plant has leaves at different ages, particular emphasis was given to the response of individual leaves influenced by nutrient solutions of different ionic strength. Nitrate content in petiole sap was much higher in older leaves than in younger leaves when grown in full strength of Cooper's nutrient solution. This difference in concentration, however, diminished in the plants grown in nutrient solution of quarter strength. Interestingly, nitrate concentration in the petiole became higher in new leaves when nitrogen deficiencies were being recovered. In contrast, NRA and total nitrogen content in the leaf blades were higher in younger leaves regardless of ionic strength. It was possible to detect the increase in nitrate concentration of petiole sap within 2–3 days of application of nitrate to cucumber plants grown in nitrate-deficient solutions.

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