ISHS Acta Horticulturae 394: Plant Bioregulators in Horticulture
PHENYLUREA DERIVATIVES FOR MICROPROPAGATION OF GARLIC MERISTEMS |
| Authors: | V. Rossi, F. Marani, A.L. Rabiti |
| Keywords: | Garlic, Meristem culture, Micropropagation, Phenylurea derivatives |
| Abstract:
Phenylurea derivatives, thidiazuron (TDZ) and N-phenyl-N'-(2-chloro-4-pyridyl) urea (4 PU-30), were added to the basal culture medium (B5) at different concentrations (0.1, 0.5, 1 mg/l) for three subcultures, in order to increase the proliferation rate of garlic shoots derived from meristem-tip culture. The activity of these compounds was compared with 0.1 and 3 mg/l BAP, previously applied in garlic meristem micropropagation. The results indicate that TDZ is much more effective than 4 PU-30 and BAP. 4 PU-30 did not induce proliferation but caused necrosis in the few shoots that formed. The proliferation rate was 1:4.5 for TDZ and 1:1.25 for BAP. At the highest TDZ concentration (1 mg/l) the increase in shoot number was associated with a progressive decrease in shoot size although rooting was normal. | |
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