ISHS Acta Horticulturae 394: Plant Bioregulators in Horticulture
ETHYLENE PRODUCTION AND ACC OXIDASE ACTIVITY IN BROCCOLI FLOWER BUDS AND THE EFFECT OF ENDOGENOUS ETHYLENE ON THEIR SENESCENCE |
| Authors: | H. Hyodo, S. Morozumi, C. Kato, K. Tanaka, H. Terai |
| Keywords: | ACC (1-aminocyclopropane-1-carboxylic acid) oxidase, Brassica oleracea, Broccoli, Ethylene, Flower buds, Senescence |
| Abstract:
Broccoli flower buds (Brassica oleracea L. Italica group) rapidly senesce during storage at 20°C in ambient atmosphere as evidenced by a rapid degradation of chlorophyll and yellowing of flower buds. The rate of ethylene production increased during senescence. The progress of senescence was greatly reduced in the presence of NBD (2,5-norbornadiene), suggesting that ethylene is associated with the development of senescence of broccoli flower buds. The progress of senescence was strongly retarded by allyl isothiocyanate. ACC (1-aminocyclopropane-1-carboxylic acid) oxidase activity sharply increased during senescence to reach a maximum and declined to a low level. ACC oxidase was extracted from flower buds and partially purified by ammonium sulfate fractionation and Sephadex G-25 column chromatography. The enzyme activity required Fe2+, HCO3- (CO2) and ascorbate as cofactors for the oxidation of ACC to ethylene. | |
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