ISHS Acta Horticulturae 393: Environmental Effects and their Control in Plant Tissue Culture
EFFECT OF DISSOLVED OXYGEN CONCENTRATION ON CARROT SOMATIC EMBRYO PRODUCTION |
| Authors: | K. Kurata, E. Fukuda |
| Keywords: | carrot, Daucus carota, dissolved oxygen, somatic embryogenesis, shake culture |
| Abstract:
Effects of dissolved oxygen (DO) concentrations on somatic embryo formation were investigated using carrot as a model plant. DO in shake culture, using flasks, were monitored and controlled by passing pure oxygen or nitrogen gas through boxes in which flasks were mounted. DO of 2.0 ppm (63μmol/ l= 24% of the saturation level) was most favorable for producing embryos capable of converting into normal plantlets. DO concentrations above 10.3 ppm (322 μmol/ l = 125% of the saturation level) did not produce any embryos. However, variations in the number of embryos produced in each flask of the same treatment were not small enough to statistically confirm the results. | |
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