ISHS Acta Horticulturae 393: Environmental Effects and their Control in Plant Tissue Culture
THE ROLE OF THE GAS PHASE IN IN VITRO CULTURE OF PRUNUS AVIUM SHOOTS |
| Authors: | B. Righetti, T.A. Thorpe, D.M. Reid, A. Rotondi |
| Keywords: | carbon dioxyde, micropropagation, oxygen, Prunus avium |
| Abstract:
Shoots of Prunus avium grown in vitro showed a clear morphological and physiological response to different CO2 and O2 concentrations: 0%CO2-21%O2, 0.09%CO2-8%O2, 0.034%CO2-21%O2. After 30 days shoots grown at 8% O2 and 0.09% CO2 showed higher fresh weight, higher % dry matter, higher lignification, lower chlorophyll content with anthocianins as compared with shoots grown at 0% and 0.034%CO2 respectively. In plantlets grown in these conditions the ratio between chlorophyll a and b also changed as compared with shoots grown at 0.034%CO2-21%O2. Furthermore shoots grown at 0% CO2 were pale green colour during the first 15 days of culture followed by a remarkable regreening and the growth rate was always lower than in cultures enriched with CO2. | |
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