ISHS Acta Horticulturae 393: Environmental Effects and their Control in Plant Tissue Culture
DEVELOPMENT OF PHOTOAUTOTROPHIC PLANT TISSUE CULTURE SYSTEM USING CO2 FROM SHIITAKE MUSHROOM |
| Authors: | Y. Kitaya, K. Sakami, T. Kozai |
| Keywords: | CO2, mushroom, photoautotrophic culture, photosynthesis, plant tissue culture, respiration |
| Abstract:
Growth characteristics were compared for plantlets cultured photoautotrophically with and without CO2 produced by respiration of mycelia of shiitake mushroom (Lentinus edodes Sing.). Plantlets were cultured on a sugar-free medium in plant culture vessels at photosynthetic photon flux density of 90 μmol m-2 s-1 and air temperature of 23–25°C. In the treatment with CO2 from mushroom, a plant culture chamber for plant culture vessels was combined tightly with a mushroom culture chamber. CO2 concentration in the plant culture chamber was controlled at about 1000 μmol mo1-1. CO2 concentrations inside the plant culture vessels during the photoperiod were 300–600 μmol mol-1 with CO2 from mushroom and 100 μmol mol-1 without CO2 from mushroom. Dry weights of mint (Mentha rotundifolia L. Huds. cv. Apple mint) and potato (Solanum tuberosum L. cv. Benimaru) plantlets cultured with CO2 from mushroom for 17 days were 1.5 and 1.3 times, respectively, those without CO2 from mushroom. | |
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