ISHS Acta Horticulturae 393: Environmental Effects and their Control in Plant Tissue Culture
EFFECTS OF RED AND FAR-RED LIGHT ON THE GROWTH AND MORPHOLOGY OF POTATO PLANTLETS IN VITRO: USING LIGHT EMITTING DIODE AS A LIGHT SOURCE FOR MICROPROPAGATION. |
| Authors: | Y. Miyashita, Y. Kitaya, T. Kozai, T. Kimura |
| Keywords: | far-red, light emitting diode, plant tissue culture, potato, red |
| Abstract:
Red (peak wavelength: 660 nm) light emitting diodes (LEDs), far-red (peak wavelength: 730 nm) LEDs, white fluorescent lamps and blue fluorescent lamps were used as light sources. Photosynthetic photon flux density (PPFD) on the empty culture shelf was adjusted at 100 μmol m-2 s-1 in all the treatments. Red photon flux density (R) and far-red photon flux density (FR) were modified, respectively, in ranges between 10 and 100 μmol m-2 s-1, and between 2 and 50 μmol m-2 s-1. Blue photon flux density was adjusted at 10 μmol m-2 s-1 in all the treatments except for the treatment with R of 100 μmol m-2 s-1. Potato plantlets were cultured on Murashige and Skoog sugar-free agar medium at air temperatures of 24/20°C (photoperiod/dark period), and a photoperiod of 16 h d-1. Shoot length increased with increasing FR/PPFD ratio when R/PPFD ratio was 0.1–0.5. Shoot length was greatest, and dry weight and leaf area were smallest with R/PPFD ratio of 1.0. R/PPFD ratio and FR/PPFD ratio affected the growth, morphology and photosynthesis of the plantlets. Possible application of LEDs to control the growth, morphology and photosynthesis of the plantlets in vitro is discussed. | |
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