ISHS Acta Horticulturae 393: Environmental Effects and their Control in Plant Tissue Culture
EFFECTS OF CO2 ENRICHMENT AND SUPPORTING MATERIAL IN VITRO ON PHOTOAUTOTROPHIC GROWTH OF EUCALYPTUS PLANTLETS IN VITRO AND EX VITRO: ANATOMICAL COMPARISONS |
| Authors: | C. Kirdmanee, Y. Kitaya, T. Kozai |
| Keywords: | CO2 enrichment, Eucalyptus, net photosynthetic rate, palisade layer, stomata, survival percentage |
| Abstract:
This study examined the leaf anatomy and net photosynthetic rate of Eucalyptus camaldulensis cultured photoautotrophically in vitro with different types of supporting materials (agar matrix, Gelrite matrix, plastic net and vermiculite) under CO2 non-enriched or CO2 enriched conditions. Subsequent survival of plantlets ex vitro was evaluated. The palisade layer thickness, palisade layer index {(Palisade layer thickness/leaf thickness)x100}, stomatal density, net photosynthetic rate in vitro and survival percentage ex vitro were significantly greater under the CO2 enriched than under the CO2 non-enriched conditions. The palisade layer index, net photosynthetic rate in vitro and survival percentage ex vitro were the highest in the vermiculite followed by the plastic net, Gelrite matrix and agar matrix (in descending order) under either the CO2 non-enriched or CO2 enriched conditions. Low stomatal density was found in the plantlets cultured in the plastic net. | |
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