ISHS Acta Horticulturae 393: Environmental Effects and their Control in Plant Tissue Culture
LOW TEMPERATURE STORAGE FOR PRODUCTION MANAGEMENT OF IN-VITRO PLANTS: EFFECTS OF AIR TEMPERATURE AND LIGHT INTENSITY ON PRESERVATION OF PLANTLET DRY WEIGHT AND QUALITY DURING STORAGE. |
| Authors: | C. Kubota, G. Niu, T. Kozai |
| Keywords: | chlorophyll fluorescence, light compensation point, micropropagation |
| Abstract:
Broccoli (Brassica oleracea L. cv. Ryokurei) seedlings were germinated aseptically and cultured photoautotrophically in vitro for 3 weeks at 23°C air temperature and 160 μmol· m-2· s-1 photosynthetic photon flux density (PPFD), then stored at 5°, 10°, or 15°C under continuous illumination at 2 or 5 μmol· m-2· s-1 PPFD. Dry weight, photosynthetic and regrowth ability of plantlets were best preserved at 5°C under 2 μmol· m-2· s-1 PPFD. At 10° and 15°C, photosynthetic and regrowth ability of plantlets after storage were higher under 5 than 2 μmol· m-2· s-1 PPFD, and decreased as air temperature or duration of storage increased. Under 5 μmol· m-2· s-1 PPFD, plantlets elongated at 10° and 15°C during storage. Dry weight of plantlets at 10°C under 5 μmol· m-2· s-1 PPFD increased during storage. These results suggest that proper combinations of air temperature and PPFD during storage would contribute to minimizing increase/decrease in dry weight and preserving photosynthetic and regrowth ability of plantlets. | |
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