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ISHS Acta Horticulturae 393: Environmental Effects and their Control in Plant Tissue Culture

ENVIRONMENTAL EFFECTS AND THEIR CONTROL IN PLANT TISSUE CULTURE - OVERVIEW

Author:   R.H. Zimmerman
Keywords:   light, medium, water relations
Abstract:
Plant tissue culture applications, both scientific and commercial, have increased at a rapid rate in recent years. Numerous species of plants are now cultured in vitro for many uses, e.g. propagation, secondary products, genetic transformation, physiological studies and germplasm conservation. As experience has been gained in culturing diverse genotypes, the influence that the total culture environment has on successfully growing organs, tissues or cells becomes more and more apparent. Early research centered mainly on the nutrient composition of the medium and the appropriate type, concentration and balance of growth regulators. Now, the significant effects on culture growth of other factors, including the gases and water relations in the culture vessel, all aspects of light, and the culture vessel itself, are recognized. The progress made in identifying, measuring, modeling and controlling the many variables that impact upon the growth of plant tissues in vitro will be described in this symposium.

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