ISHS Acta Horticulturae 392: Genetic Improvement of Horticultural Crops by Biotechnology
S-RNASE LIKE GENOMIC SEQUENCES IN APPLE FOR DNA FINGERPRINTING |
| Authors: | S. Matsumoto, N. Hoshi, T. Tsuchiya, J. Soejima, S. Komori, S. Ejiri |
| Keywords: | apple, Malus, Self-incompatibility, S-RNase, Cultivar identification, Restriction fragment length polymorphism |
| Abstract:
DNA fingerprinting using genomic DNA sequences of apple with similarity to S-RNase or S-like RNase genes have been investigated for species and cultivar identification. Two amplified DNA fragments named A-2 (312 bp) and A-4 (294 bp) from apple genomic DNA contained DNA sequences indicative of intron splice sites. Both of the deduced amino-acid sequences showed 25 % to 66 % identity to the S-RNases, S-like RNases, and fungal RNases. Southern blot analyses showed that the A-2 and A-4 probes produce distinguishable RFLP patterns between self-compatible and self-incompatible materials. Moreover, the A-2 and A-4 fragments gave different RFLP patterns amongst Malus species and cultivars of Malus domestica. | |
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