ISHS Acta Horticulturae 392: Genetic Improvement of Horticultural Crops by Biotechnology
REGULATION OF GENE EXPRESSION BY 2,4-D DURING ORGANOGENESIS IN GLADIOLUS CV. TOPAZ CALLUS |
| Authors: | M.S. Kang, S.G. Suh, I. Maruta, K.W. Kim |
| Keywords: | Gladiolus, Callus, 2,4-D, Organogenesis, mRNA, In vitro translation, IEF-SDS· PAGE |
| Abstract:
Calli were induced from gladiolus cormel tissue, subcultured on Murashige and Skoog (MS) medium with 0.5mg/l 2,4-D and differentiated to root on 2,4-D free MS medium. Root formation was observed 6 days after transfer to 2,4-D free MS medium. Changes of protein patterns and in vitro translation products were observed during root formation by isoelectric focusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS·PAGE). Over 300 proteins were separated by IEF-SDS· PAGE and qualitative and quantitative changes were observed in proteins having molecular weights from 26kDa to 84Da. Three proteins were de novo synthesized 6 days after callus culture for root formation. Five newly synthesized proteins of low molecular weights were observed in in vitro translation products of mRNA prepared from root-forming callus. | |
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