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ISHS Acta Horticulturae 392: Genetic Improvement of Horticultural Crops by Biotechnology

APPLICATION OF ANTHER CULTURE AND ISOLATED MICROSPORE CULTURE TO VEGETABLE CROP IMPROVEMENT

Authors:   M.Q. Cao, Y. Li, F. Liu, T. Jiang, G.S. Liu
Keywords:   Androgenesis, Anther culture, Isolated microspore culture, Doubled haploid (DH), Genetic improvement, Vegetable crops, Chinese cabbage (Brassica rapa var. pekinensis, B. r. var. chinensis)
Abstract:
In vitro anther culture used for obtaining haploid plants is now routinely used for improving some vegetable crops such as asparagus, sweet pepper, eggplant, watermelon and Brassica vegetables. Isolated microspore culture has been successfully carried out with some Brassica vegetables such as cabbage, broccoli and Chinese cabbage-petsai and pakchoi. Most genotypes respond better to isolated microspore culture and embryo yield is generally higher than with anther culture, therefore isolated microspore culture has been preferred as a breeding tool and as an experimental system for various genetic manipulations. A Chinese cabbage breeding program using this technique is underway in the BVRC.
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