ISHS Acta Horticulturae 392: Genetic Improvement of Horticultural Crops by Biotechnology
COMPARISON OF PROMOTER-DEPENDENT EXPRESSION OF β-GLUCURONIDASE GENES IN TRANSGENIC TORENIA PLANTS |
| Authors: | R. Aida, M. Shibata |
| Keywords: | Torenia fournieri, Cauliflower mosaic virus-35S promoter, Pathogenesis-related protein gene promoter, β-glucuronidase, Genetic transformation, Gene expression, Salicylic acid |
| Abstract:
Promoter-dependent expression of introduced  -glucuronidase (GUS) gene in the transgenic torenia plants (Torenia fournieri) is reported.
GUS chimeric genes used in this experiment were, 1: coding region of GUS gene containing an intron fused with cauliflower mosaic virus-35S (CaMV35S) promoter (35S/Intron-GUS), 2: coding region of GUS gene fused with revised CaMV35S promoter having the omega arrangement derived from tobacco mosaic virus (E12Ω/GUS), and 3: coding region of GUS gene fused with PR1a promoter derived from pathogenesis-related la protein gene of tobacco (PR1a/GUS). These GUS chimeric genes were introduced into torenia plants by Agrobacterium-mediated transformation.
The plants harbouring the E12Ω/GUS showed higher GUS activity than the plants harbouring the 35S/Intron-GUS. All plants harbouring the E12 Ω /GUS showed GUS activity more than 10 nanomoles 4-methylumbelliferone/mg protein/30min. at 37°C. Though only 37 % of the plants harbouring the 35S/Intron-GUS showed the activity of more than 10. The expression level in the plants harbouring the PR1a/GUS was increased markedly by salicylic acid (SA) treatment.
The 17.7 times higher GUS activity was observed in the selected 10 plants harbouring the PR1a/GUS by treatment with 0.5 mM SA for 3 days when compared with that of non-treated.
Usefulness of these promoters for controlling expression of introduced genes was shown in torenia plants, as in the case of other plants.
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