ISHS Acta Horticulturae 392: Genetic Improvement of Horticultural Crops by Biotechnology
DIRECT GENE TRANSFER TO TALL FESCUE (FESTUCA ARUNDINACEA SCHREB.) BY PARTICLE BOMBARDMENT |
| Authors: | T. Takamizo, T. Hagio, M. Fujimori, M. Ugaki, T. Hirabayashi |
| Keywords: | Tall fescue, Particle bonbardment, Direct gene transfer, GUS, Transformed callus |
| Abstract:
Tall fescue (Festuca arundinacea Schreb.) suspension culture cells were bombarded by the particle gun with plasmids carrying the  -glucuronidase (GUS) reporter gene.
Various parameters affecting transient GUS expression were investigated.
As for micro carrier, tungsten was found to be superior to gold, when 1.6μ particles were used.
A bombardment pressure of 1300psi was superior to one of 1100psi.
Tall fescue suspension culture showed higher GUS expression levels than meadow fescue and Italian ryegrass suspension cells.
High expression levels were observed with improved constructs originating form a Geminivirus-derived vector or containing intron or leader sequences.
Tall fescue suspension culture cells were also bombarded with the Geminivirus-derived constructs (pWI-K6) carrying the Neomycin phsophotransferase II gene (NPT II). Putative transformed calli were obtained by selecting the bombarded cells on MS medium supplemented with 50 mg/l Geneticin. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
