ISHS Acta Horticulturae 392: Genetic Improvement of Horticultural Crops by Biotechnology
AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF CHRYSANTHEMUM |
| Authors: | S. Fukai, J. De Jong, W. Rademaker |
| Keywords: | Agrobacterium tumefaciens, Chrysanthemum, Genetic transformation |
| Abstract:
Transgenic chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) plants were obtained using Agrobacterium tumefaciens strain AGLO containing the binary vector pMOG410 carrying the neomycine phosphotransferase II and  -glucuronidase-intron genes.
Genotypic differences in regeneration potential and sensitivity to kanamycin affected the final efficiencies of transformation.
Variations in phenotypical features were observed among clones in which the presence of both genes in their genome was confirmed by PCR.
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