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ISHS Acta Horticulturae 390: Internat. Symposium on Medicinal and Aromatic Plants

PRODUCTION OF TAXOL FROM IN VITRO CELL CULTURE OF TAXUS CUSPIDATA

Authors:   J.H. Park, C.H. Kim, I.S. Chung, S.W. Lee
Keywords:   taxol, phosphate, nitrogen, immobilization
Abstract:
The effects of nitrogen, phosphate and cell immobilization on growth and taxol production were investigated using cell culture of Taxus cuspidata. The ratio of nitrate to ammonium was found to be an important parameter. Reducing nitrogen or phosphate concentration retarded cell growth, but improved taxol formation noticeably. Our findings also confirmed that cell immobilization in Ca-alginate beads increased taxol formation.

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