ISHS Acta Horticulturae 390: Internat. Symposium on Medicinal and Aromatic Plants
EFFECT OF PRECURSOR AND POTASSIUM NITRATE ON INDIRUBIN PRODUCTION IN SUSPENSION CULTURE OF POLYGONUM TINCTORIUM |
| Authors: | T. Kim, G.W. Bae, I.S. Chung, H.J. Lee, Y.A. Chae |
| Keywords: | indirubin, fed-batch culture, air-lift bioreactor, indole |
| Abstract:
Effect of precursor and potassium nitrate on the growth of Polygonum tinctorium and indirubin formation was investigated using shake flasks and air-lift bioreactors. Cell growth was impaired by precursor addition at the level higher than 2 mM indole. Indirubin formation increased when 20 mM of indole was added at the mid-point of exponential phase of cells. In an air-lift bioreactor using nitrate depleted media fed-batch culture increased indirubin production by 40%, compared with the control. | |
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