ISHS Acta Horticulturae 387: III International Protea Research Symposium
MICROPROPAGATION OF PROTEA REPENS |
| Author: | B.A. Rugge |
| Abstract:
Multinodal explants (3 – 5 nodes) of Protea repens cv. Embers, were established on half-strength Murashige and Skoog medium supplemented with 1 mg.l-1 benzyladenine. Treating actively growing shoots on field grown mother plants with 200 mg.l-1 benzyladenine, significantly reduced browning and promoted bud sprouting in vitro. Treating mother plants with captafol (Difolatan, 1 g.l-1) and iprodione (Rovral flo, 1 ml.l-1), reduced the contamination rate during establishment to 14% compared to 90% of the control. Bud break in vitro was increased by the addition of gibberellic acid at 6 mg.l-1. Somatic embryos developed from the base of the shootlet throughout the multiplication stage. | |
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