ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
IDENTIFICATION OF A FLEXEOUS VIRUS ASSOCIATED WITH CHERRY TWISTED LEAF DISEASE |
| Authors: | D. James, W.E. Howell, R.R. Martin |
| Abstract:
Cherry twisted leaf (ChTL) associated double stranded RNA (dsRNA) was isolated from the petioles of symptomatic Bing cherry plants. The ChTL-dsRNA was isolated by two cycles of CF11 column chromatography, T1 RNase and DNase treated, and agarose gel purified. Oligonucleotide primers based on conserved closterovirus sequences were used in RT-PCR to generate a ChTL-specific 600 bp fragment. This fragment was digoxigenin labelled and used successfully to detect cloned cDNA produced using dsRNA from the putative ChTL-associated virus in Nicotiana occidentalis. Positive results were also obtained when blots containing total RNA from ChTL-affected Bing cherry, and from N. occidentalis infected with the putative ChTL virus were hybridized with the digoxigenin labelled probe. | |
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