ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
VIROID-LIKE RNAS ASSOCIATED WITH SOME APRICOT FRUIT DISORDERS |
| Authors: | F. Di Serio, A. Ragozzino, D. Alioto |
| Abstract:
In Campania two apricot diseases of the cultivars "Bulida" and "Tyrinthos" on Myrabolan rootstocks have been recognized. The "Bulida" disease, characterized by the presence of brown-reddish round spots and/or concentric rings, is graft-transmissible and resembles apricot ring pox described in the U.S.A. and Canada. The diseased "Tyrinthos" trees produce deformed fruits showing depressed green spots and few peculiar symptoms on the leaves. Transmission studies, started in 1992 by grafting diseased bark-chips onto 2 years old "Tyrinthos" seedlings, are in progress. In spite of the constant detection of apple chlorotic leaf spot virus (CLSV) in the diseased trees of both cultivars, further investigations were carried out on the etiology of the disorders. In fact, five "Builda" seedlings infected in 1987 with CLSV, isolated on Chenopodium quinoa, by approach grafting and/or inserting C. quinoa tissues underneath the bark, produced in 1992 fruits showing only reddish lines and rings. Using nucleic acid extraction and viroid RNAs detection procedure of Flores et al., (1990) viroid-like RNAs were detected in leaves of diseased "Builda" and "Tyrinthos" trees. The electrophoretic mobility of these RNAs under denaturing conditions were similar to that of hop stunt viroid (HSVd) recently detected in apricot. Our results suggest that the described disorders could be caused by the simultaneous infection of CLSV and viroid. | |
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